Description
Boster’s mouse TEK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for TEK has been precoated onto 96-well plates. Standards(NSO, A23-K744) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TEK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TEK amount of sample captured in plate.
Synonyms: Angiopoietin-1 receptor|CD202b|CD202b antigen|EC 2.7.10.1|hTIE2|p140 TEK|soluble TIE2|variant 1|soluble TIE2 variant 2|Tek|TEK tyrosine kinase, endothelial|TIE 2|TIE2_HUMAN|Tunica interna endothelial cell kinase|Tyrosine-protein kinase receptor TEK|Tyrosine-protein kinase receptor TIE 2|Tyrosine-protein kinase receptor TIE-2|venous malformations, multiple cutaneous and mucosal|VMCM|VMCM1
Species: Mouse TEK
Assay: 125pg/ml-8000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse TEK
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, A23-K744
Gene name: Angiopoietin-1 receptor / TEK tyrosine kinase, endothelial
Protein name: Angiopoietin-1 receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|158|1232|4836|145|1295|4385
Standard deviation|7.9|66.52|203.11|7.97|90.65|210.48
CV(%)|5%|5.4%|4.2%|5.5%|7%|4.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 158 | 174 | 173 | 164 | 167 | 6.6 | 3.9% |
| Sample 2 | 1232 | 1223 | 1255 | 1137 | 1211 | 44.7 | 3.6% |
| Sample 3 | 4836 | 4677 | 4895 | 5026 | 4858 | 125.33 | 2.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0976-CAP|96-well plate precoated with anti- mouse TEK antibody|1
EK0976-ST|Lyophilized recombinant mouse TEK standard|10ng/tube×2
EK0976-DA|Biotinylated anti- mouse TEK antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
