Description
Boster’s mouse SCF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SCF has been precoated onto 96-well plates. Standards(E.coli, K26-A190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SCF amount of sample captured in plate.
Synonyms: C kit ligand|_x000D_Ckit ligand|_x000D_Kit ligand|_x000D__x000D__x000D_Kitl|_x000D_KITLG|_x000D_KL 1|_x000D_KL1|_x000D_Mast cell growth factor|_x000D_MGF|_x000D_SF|_x000D_SHEP7|_x000D_Steel factor|_x000D_Stem cell factor|_x000D_Stem cell factor precursor
Species: Mouse SCF
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse SCF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: EDTA
Immunogen: E.coli, K26-A190
Gene name: Kit ligand / KIT ligand
Protein name: Kit ligand
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|75|190|820|81|186|861
Standard deviation|5.25|10.26|42.64|6.96|12.46|45.63
CV(%)|7%|5.4%|5.2%|8.6%|6.7%|5.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 75 | 88 | 85 | 89 | 84 | 5.53 | 6.5% |
| Sample 2 | 190 | 214 | 186 | 211 | 200 | 12.37 | 6.1% |
| Sample 3 | 820 | 795 | 798 | 947 | 840 | 62.52 | 7.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0509-CAP|96-well plate precoated with anti- mouse SCF antibody|1
EK0509-ST|Lyophilized recombinant mouse SCF standard|10ng/tubex2
EK0509-DA|Biotinylated anti- mouse SCF antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
