Description
Boster’s mouse Resistin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Resistin has been precoated onto 96-well plates. Standards(E.coli, S21-S114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Resistin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Resistin amount of sample captured in plate.
Synonyms: Adipose tissue specific secretory factor|_x000D_Adipose tissue-specific secretory factor|_x000D_ADSF|_x000D__x000D__x000D_C/EBP epsilon regulated myeloid specific secreted cysteine rich protein|_x000D_C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1|_x000D_C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein|_x000D_Cysteine rich secreted protein A12 alpha like 2|_x000D_Cysteine rich secreted protein FIZZ3|_x000D_Cysteine-rich secreted protein A12-alpha-like 2|_x000D_Cysteine-rich secreted protein FIZZ3|_x000D_FIZZ 3|_x000D_FIZZ3|_x000D_Found in inflammatory zone 3|_x000D_HXCP 1|_x000D_HXCP1|_x000D_MGC126603|_x000D_MGC126609|_x000D_PRO1199|_x000D_Resistin|_x000D_Resistin delta2|_x000D_RETN|_x000D_RETN 1|_x000D_RETN_HUMAN|_x000D_RETN1|_x000D_UNQ407|_x000D_XCP 1|_x000D_XCP1
Species: Mouse Resistin
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant mouse Resistin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: E.coli, S21-S114
Gene name: Resistin / resistin
Protein name: Resistin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|77|245|929|80|252|861
Standard deviation|5.92|13.23|57.59|7.28|17.64|69.74
CV(%)|7.7%|5.4%|6.2%|9.1%|7%|8.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 77 | 78 | 88 | 84 | 81 | 4.49 | 5.5% |
| Sample 2 | 245 | 263 | 267 | 252 | 256 | 8.72 | 3.4% |
| Sample 3 | 929 | 803 | 900 | 906 | 884 | 48.28 | 5.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0582-CAP|96-well plate precoated with anti- mouse Resistin antibody|1
EK0582-ST|Lyophilized recombinant mouse Resistin standard|10ng/tubex2
EK0582-DA|Biotinylated anti- mouse Resistin antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
