Description
Boster’s mouse PLA2G2A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLA2G2A has been precoated onto 96-well plates. Standards(Expression system for standard: sf21, Immunogen sequence: N22-C146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLA2G2A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Phospholipase A2, membrane associated; Enhancing factor; EF; GIIC sPLA2; Group IIA phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A; Pla2g2a
Species: Mouse PLA2G2A
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse PLA2G2A
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: sf21, N22-C146
Gene name: Phospholipase A2, membrane associated / phospholipase A2, group IIA (platelets, synovial fluid)
Protein name: Phospholipase A2, membrane associated
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|56|234|938|58|242|951
Standard deviation|2.69|13.34|43.15|2.96|14.28|60.86
CV(%)|4.8%|5.7%|4.6%|5.1%|5.9%|6.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 56 | 59 | 55 | 62 | 58 | 2.73 | 4.7% |
| Sample 2 | 234 | 232 | 234 | 234 | 233 | 0.86 | 0.3% |
| Sample 3 | 938 | 983 | 1055 | 1031 | 1001 | 45.01 | 4.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1945-CAP|96-well plate precoated with anti- mouse PLA2G2A antibody|1
EK1945-ST|Lyophilized recombinant mouse PLA2G2A standard|10ng/tube×2
EK1945-DA|Biotinylated anti- mouse PLA2G2A antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
