Description
Boster’s mouse Periostin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Periostin has been precoated onto 96-well plates. Standards(NSO, N24-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Periostin amount of sample captured in plate.
Synonyms: OSF-2|Osteoblast-specific factor 2|Periostin|PN|POSTN|POSTN_HUMAN
Species: mouse Periostin
Assay: 93.7pg/ml-6000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse Periostin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, N24-Q836
Gene name: Periostin / periostin, osteoblast specific factor
Protein name: Periostin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|109|795|2510|119|740|2598
Standard deviation|6.97|53.26|110.44|8.33|62.9|119.5
CV(%)|6.4%|6.7%|4.4%|7%|8.5%|4.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 109 | 109 | 111 | 107 | 109 | 1.41 | 1.2% |
| Sample 2 | 795 | 840 | 784 | 883 | 825 | 39.27 | 4.7% |
| Sample 3 | 2510 | 2434 | 2494 | 2308 | 2436 | 79.41 | 3.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1187-CAP|96-well plate precoated with anti- mouse Periostin antibody|1
EK1187-ST|Lyophilized recombinant mouse Periostin standard|10ng/tube×2
EK1187-DA|Biotinylated anti- mouse Periostin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
