Description
Boster’s mouse Neuropilin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Neuropilin-2 has been precoated onto 96-well plates. Standards(NSO, Q23-D863) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Neuropilin-2 amount of sample captured in plate.
Synonyms: MGC126574|Neuropilin 2|Neuropilin-2|Neuropilin2|NP 2|NP2|NPN 2|NPN2|NRP 2|Nrp2|NRP2_HUMAN|PRO2714|Receptor for VEGF165 and semaphorins class3|Vascular endothelial cell growth factor 165 receptor 2|Vascular endothelial growth factor 165 receptor 2|VEGF1265R2|VEGF165R2
Species: mouse Neuropilin-2
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse Neuropilin-2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, Q23-D863
Gene name: Neuropilin-2 / neuropilin 2
Protein name: Neuropilin-2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|267|1352|5179|243|1366|4747
Standard deviation|17.62|66.24|346.99|16.52|77.86|394
CV(%)|6.6%|4.9%|6.7%|6.8%|5.7%|8.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 267 | 276 | 277 | 301 | 280 | 12.59 | 4.4% |
| Sample 2 | 1352 | 1396 | 1389 | 1292 | 1357 | 41.21 | 3% |
| Sample 3 | 5179 | 5231 | 5457 | 4899 | 5191 | 198.59 | 3.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1344-CAP|96-well plate precoated with anti- mouse Neuropilin-2 antibody|1
EK1344-ST|Lyophilized recombinant mouse Neuropilin-2 standard|10ng/tube×2
EK1344-DA|Biotinylated anti- mouse Neuropilin-2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
