Description
Boster’s mouse MER ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MER has been precoated onto 96-well plates. Standards(sf21, E23-F498) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MER is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MER amount of sample captured in plate.
Synonyms: C mer|_x000D_c mer proto oncogene tyrosine kinase|_x000D_MER receptor tyrosine kinase|_x000D__x000D__x000D_Mertk|_x000D_MERTK_HUMAN|_x000D_Proto-oncogene c-Mer|_x000D_Receptor tyrosine kinase MerTK|_x000D_RP38|_x000D_STK kinase|_x000D_Tyrosine-protein kinase Mer
Species: Mouse MER
Assay: 78pg/ml-5000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse MER
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: sf21, E23-F498
Gene name: Tyrosine-protein kinase Mer / c-mer proto-oncogene tyrosine kinase
Protein name: Tyrosine-protein kinase Mer
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|109|571|2250|114|562|2147
Standard deviation|6.86|30.83|132.75|8.55|39.34|145.99
CV(%)|6.3%|5.4%|5.9%|7.5%|7%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 109 | 127 | 129 | 123 | 122 | 7.81 | 6.4% |
| Sample 2 | 571 | 619 | 574 | 585 | 587 | 19.05 | 3.2% |
| Sample 3 | 2250 | 2045 | 2170 | 2458 | 2230 | 150.17 | 6.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0811-CAP|96-well plate precoated with anti- mouse MER antibody|1
EK0811-ST|Lyophilized recombinant mouse MER standard|10ng/tube×2
EK0811-DA|Biotinylated anti- mouse MER antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
