Description
Boster’s mouse Leptin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Leptin has been precoated onto 96-well plates. Standards(E.coli, V22-C167) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Leptin amount of sample captured in plate.
Synonyms: FLJ94114|_x000D_LEP|_x000D_LEP_HUMAN|_x000D__x000D__x000D_Leptin|_x000D_Leptin Murine Obesity Homolog|_x000D_Leptin Precursor Obesity Factor|_x000D_OB|_x000D_Obese protein|_x000D_Obesity factor|_x000D_Obesity homolog mouse |_x000D_Obesity murine homolog leptin|_x000D_OBS
Species: Mouse Leptin
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse Leptin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, V22-C167
Gene name: Leptin / leptin
Protein name: Leptin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|104|827|1634|104|819|1564
Standard deviation|7.28|40.52|114.38|7.9|40.13|131.37
CV(%)|7%|4.9%|7%|7.6%|4.9%|8.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 104 | 106 | 98 | 112 | 105 | 5 | 4.7% |
| Sample 2 | 827 | 838 | 789 | 789 | 810 | 22.09 | 2.7% |
| Sample 3 | 1634 | 1711 | 1569 | 1673 | 1646 | 52.49 | 3.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0438-CAP|96-well plate precoated with anti- mouse Leptin antibody|1
EK0438-ST|Lyophilized recombinant mouse Leptin standard|10ng/tubex2
EK0438-DA|Biotinylated anti- mouse Leptin antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
