Description
Boster’s mouse IL15RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for IL15RA has been precoated onto 96-well plates. Standards(NSO, G33-K205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL15RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL15RA amount of sample captured in plate.
Synonyms: AA690181|CD215|I15RA_HUMAN|IL 15R alpha|IL-15 receptor subunit alpha|IL-15R-alpha|IL-15RA|IL15RA|Interleukin 15 receptor alpha|Interleukin 15 receptor subunit alpha|MGC104179|sIL-15 receptor subunit alpha|sIL-15R-alpha|sIL-15RA|Soluble interleukin 15 receptor subunit alpha|Soluble interleukin-15 receptor subunit alpha
Species: mouse IL15RA
Assay: 125pg/ml-8000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse IL15RA
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, G33-K205
Gene name: Interleukin-15 receptor subunit alpha / interleukin 15 receptor, alpha
Protein name: Interleukin-15 receptor subunit alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|181|1121|6129|165|1015|6536
Standard deviation|12.48|77.34|306.45|11.55|85.26|326.8
CV(%)|6.9%|6.9%|5%|7%|8.4%|5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 181 | 196 | 202 | 196 | 193 | 7.75 | 4% |
| Sample 2 | 1121 | 1352 | 1324 | 1168 | 1241 | 98.66 | 7.9% |
| Sample 3 | 6129 | 5379 | 6344 | 6290 | 6035 | 387.19 | 6.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1142-CAP|96-well plate precoated with anti- mouse IL15RA antibody|1
EK1142-ST|Lyophilized recombinant mouse IL15RA standard|10ng/tube×2
EK1142-DA|Biotinylated anti- mouse IL15RA antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
