Description
Boster’s mouse IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli, R27-Q178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1RA amount of sample captured in plate.
Synonyms: ICIL 1RA|IL1 inhibitor|ICIL-1RA|ICIL1RA|IL-1ra|IL-1RN|IL1 inhibitor|IL1F3|IL1RA_HUMAN|IL1RN|Interleukin 1 Receptor Antagonist Protein|IRAP
Species: Mouse IL-1RA
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse IL-1RA
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, R27-Q178
Gene name: Interleukin-1 receptor antagonist protein / interleukin 1 receptor antagonist
Protein name: Interleukin-1 receptor antagonist protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|99|586|1056|92|570|1109
Standard deviation|4.95|36.91|47.52|6.25|42.18|64.32
CV(%)|5%|6.3%|4.5%|6.8%|7.4%|5.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 99 | 89 | 98 | 95 | 95 | 3.89 | 4% |
| Sample 2 | 586 | 555 | 523 | 545 | 552 | 22.66 | 4.1% |
| Sample 3 | 1056 | 1070 | 1157 | 1068 | 1087 | 40.33 | 3.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0783-CAP|96-well plate precoated with anti- mouse IL-1RAantibody|1
EK0783-ST|Lyophilized recombinant mouse IL-1RA standard|10ng/tube×2
EK0783-DA|Biotinylated anti- mouse IL-1RA antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
