Mouse IGFBP5 PicoKine ELISA Kit

Brand:
Boster Bio
Storage:

For quantitative detection of mouse IGFBP5 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

 

Available on backorder

SKU: EK1183 Category:

Description

Boster’s mouse IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP5 has been precoated onto 96-well plates. Standards(NSO, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP5 amount of sample captured in plate.


Synonyms: IBP 5|_x000D_IBP-5|_x000D_IBP5|_x000D__x000D__x000D_IBP5_HUMAN|_x000D_IGF binding protein 5|_x000D_IGF BP5|_x000D_IGF-binding protein 5|_x000D_IGFBP 5|_x000D_IGFBP-5|_x000D_IGFBP5|_x000D_Insulin like growth factor binding protein 5|_x000D_Insulin-like growth factor-binding protein 5

Species: mouse IGFBP5

Assay: 156pg/ml-10000pg/ml

Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse IGFBP5

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Anticoagulant: heparin or EDTA

Immunogen: NSO, L20-E271

Gene name: Insulin-like growth factor-binding protein 5 / insulin-like growth factor binding protein 5

Protein name: Insulin-like growth factor-binding protein 5

Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)


|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|223|1270|6078|229|1195|6403
Standard deviation|16.5|71.12|255.27|21.29|76.48|332.95
CV(%)|7.4%|5.6%|4.2%|9.3%|6.4%|5.2%


Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 223 257 257 258 248 14.87 5.9%
Sample 2 1270 1408 1312 1242 1308 62.88 4.8%
Sample 3 6078 5394 5607 6353 5858 378.06 6.4%

*number of samples for each test n=16.


Catalog number|Description|Quantity
EK1183-CAP|96-well plate precoated with anti- mouse IGFBP5 antibody|1
EK1183-ST|Lyophilized recombinant mouse IGFBP5 standard|10ng/tube×2
EK1183-DA|Biotinylated anti- mouse IGFBP5 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4