Description
Boster’s mouse FAS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FAS has been precoated onto 96-well plates. Standards(sf21, Q22-R169) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FAS amount of sample captured in plate.
Synonyms: ALPS1A|APO 1|APO 1 cell surface antigen|Apo-1 antigen|APO1|Apo1 antigen|Apoptosis antigen 1|Apoptosis mediating surface antigen FAS|Apoptosis-mediating surface antigen FAS|APT1|CD95|CD95 antigen|Fas|Fas AMA|Fas antigen|FAS1|FASLG receptor|FASTM|TNF receptor superfamily member 6|TNFRSF6|TNR6_HUMAN|Tumor necrosis factor receptor superfamily member 6
Species: Mouse FAS
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <3pg/ml Specifity: Natural and recombinant mouse FAS
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: sf21, Q22-R169
Gene name: Tumor necrosis factor receptor superfamily member 6 / Fas cell surface death receptor
Protein name: Tumor necrosis factor receptor superfamily member 6
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|67|227|869|60|222|905
Standard deviation|3.35|14.07|63.43|3.66|17.76|70.59
CV(%)|5%|6.2%|7.3%|6.1%|8%|7.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 67 | 72 | 66 | 74 | 69 | 3.34 | 4.8% |
| Sample 2 | 227 | 219 | 205 | 237 | 222 | 11.7 | 5.2% |
| Sample 3 | 869 | 913 | 935 | 934 | 912 | 26.74 | 2.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0336-CAP|96-well plate precoated with anti- mouse FAS antibody|1
EK0336-ST|Lyophilized recombinant mouse FAS standard|10ng/tube×2
EK0336-DA|Biotinylated anti- mouse FAS antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
