Description
Boster’s mouse ESM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ESM1 has been precoated onto 96-well plates. Standards(NSO, W20-R184) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ESM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ESM1 amount of sample captured in plate.
Synonyms: Endothelial cell-specific molecule 1|ESM-1|Esm1|ESM1_HUMAN
Species: Mouse ESM1
Assay: 23.4pg/ml-1500pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse ESM1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, W20-R184
Gene name: Endothelial cell-specific molecule 1 / endothelial cell-specific molecule 1
Protein name: Endothelial cell-specific molecule 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|56|169|683|60|163|636
Standard deviation|3.36|10.14|52.59|4.2|12.22|56.6
CV(%)|6%|6%|7.7%|7%|7.5%|8.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 56 | 53 | 49 | 52 | 52 | 2.5 | 4.8% |
| Sample 2 | 169 | 162 | 178 | 184 | 173 | 8.4 | 4.8% |
| Sample 3 | 683 | 675 | 706 | 643 | 676 | 22.56 | 3.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0753-CAP|96-well plate precoated with anti- mouse ESM1 antibody|1
EK0753-ST|Lyophilized recombinant mouse ESM1 standard|10ng/tube×2
EK0753-DA|Biotinylated anti- mouse ESM1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
