Description
Boster’s mouse Endothelin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endothelin has been precoated onto 96-well plates. Standards (mouse peptide,C53-W73) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Endothelin amount of sample captured in plate.
Synonyms: Big endothelin 1|ET1|HDLCQ7|PPET1|Preproendothelin 1|EDN2|EDN2_HUMAN|Endothelin 2|Endothelin-2|ET 2|ET-2|ET2|PPET2|Preproendothelin 2|Preproendothelin-2|EDN3|EDN3_HUMAN|Endothelin-3|ET 3|ET-3|PPET3|Preproendothelin 3|Preproendothelin-3
Species: Mouse Endothelin
Assay: 3.9pg/ml-250pg/ml
Sensitivy: <0.5pg/ml Specifity: Natural and recombinant mouse Endothelin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated monoclonal antibody from mouse
Anticoagulant: heparin or EDTA
Immunogen: human peptide, C53-W73
Gene name: Endothelin-1 / endothelin 1
Protein name: Endothelin-1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|19|52|101|18|54|95
Standard deviation|1.25|3.69|4.04|1.33|4.15|4.94
CV(%)|6.6%|7.1%|4%|7.4%|7.7%|5.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 19 | 19 | 18 | 17 | 18 | 0.82 | 4.5% |
| Sample 2 | 52 | 52 | 61 | 59 | 56 | 4.06 | 7.2% |
| Sample 3 | 101 | 106 | 111 | 101 | 104 | 4.14 | 3.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0953-CAP|96-well plate precoated with anti- mouse Endothelin antibody|1
EK0953-ST|Lyophilized recombinant mouse Endothelin standard|1ng/tube×2
EK0953-DA|Biotinylated anti- mouse Endothelin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
