Description
Boster’s mouse DKK4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DKK4 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: L19-I221) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DKK4 amount of sample captured in plate.
Synonyms: Dickkopf 4 | Dickkopf gene 4 | Dickkopf homolog 4 | Dickkopf related protein 4 | Dickkopf4 | DKK 4 | hDkk4 | MGC129562 | MGC129563 | MGC25705 | RGD1563172 | Q9UBT3
Species: Mouse DKK4
Assay: 78pg/ml-5000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse DKK4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, L19-I221
Gene name: Dickkopf-related protein 4 / dickkopf WNT signaling pathway inhibitor 4
Protein name: Dickkopf-related protein 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|115|828|2616|104|829|2555
Standard deviation|7.13|36.43|133.41|8.21|51.39|178.85
CV(%)|6.2%|4.4%|5.1%|7.9%|6.2%|7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 115 | 112 | 113 | 121 | 115 | 3.49 | 3% |
| Sample 2 | 828 | 710 | 788 | 755 | 770 | 43.33 | 5.6% |
| Sample 3 | 2616 | 2592 | 2575 | 2681 | 2616 | 40.25 | 1.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1578-CAP|96-well plate precoated with anti- mouse DKK4 antibody|1
EK1578-ST|Lyophilized recombinant mouse DKK4 standard|10ng/tube×2
EK1578-DA|Biotinylated anti- mouse DKK4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
