Description
Boster’s mouse CXCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL16 has been precoated onto 96-well plates. Standards(E.coli, N27-P114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL16 amount of sample captured in plate.
Synonyms: 0910001K24Rik|_x000D_AV290116|_x000D_BB024863|_x000D__x000D__x000D_C X C motif chemokine 16|_x000D_Chemokine (C X C motif) ligand 16|_x000D_CXC chemokine ligand 16|_x000D_CXCLG16|_x000D_DN 491F10.6|_x000D_Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein|_x000D_SCYB16|_x000D_Small inducible cytokine B|_x000D_Small inducible cytokine B16|_x000D_Small inducible cytokine B16 precursor|_x000D_SR PSOX|_x000D_SR PSOX/CXCL16 scavenger receptor for phosphatidylserine and oxidized low density lipoprotein|_x000D_SRPSOX|_x000D_Transmembrane chemokine CXCL16|_x000D_Zinc finger, MYND-type containing 15|_x000D_Zmynd15
Species: Mouse CXCL16
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant mouse CXCL16
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, N27-P114
Gene name: C-X-C motif chemokine 16 / chemokine (C-X-C motif) ligand 16
Protein name: C-X-C motif chemokine 16
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|32|238|389|29|242|392
Standard deviation|1.28|10.71|27.61|1.24|12.34|29.79
CV(%)|4%|4.5%|7.1%|4.3%|5.1%|7.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 32 | 31 | 35 | 32 | 32 | 1.5 | 4.6% |
| Sample 2 | 238 | 241 | 246 | 235 | 240 | 4.06 | 1.6% |
| Sample 3 | 389 | 377 | 372 | 378 | 379 | 6.2 | 1.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0742-CAP|96-well plate precoated with anti- mouse CXCL16 antibody|1
EK0742-ST|Lyophilized recombinant mouse CXCL16 standard|10ng/tube×2
EK0742-DA|Biotinylated anti- mouse CXCL16 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
