Description
Boster’s mouse CD48 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD48 has been precoated onto 96-well plates. Standards(NSO, F23-S217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD48 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD48 amount of sample captured in plate.
Synonyms: Antigen CD48|B cell membrane protein|B lymphocyte activation marker BLAST 1 [Precursor]|B lymphocyte activation marker BLAST 1|B-cell activation marker|B-lymphocyte activation marker BLAST-1|BCM 1 surface antigen|BCM1|BCM1 surface antigen|BLAST 1|BLAST|BLAST1|CD 48|CD48|CD48 antigen (B cell membrane protein)|CD48 antigen|CD48 molecule|CD48 protein|CD48_HUMAN|hCD48|Leucocyte antigen MEM 102|Leukocyte antigen MEM-102|mCD48|MEM 102|MEM-102|MEM102|Signaling lymphocytic activation molecule 2|SLAM family member 2|SLAMF 2|SLAMF2|TCT.1
Species: mouse CD48
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CD48
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, F23-S217
Gene name: CD48 antigen / CD48 molecule
Protein name: CD48 antigen
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|91|816|1332|95|736|1273
Standard deviation|4|34.27|87.91|5.03|41.21|98.02
CV(%)|4.4%|4.2%|6.6%|5.3%|5.6%|7.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 91 | 79 | 95 | 86 | 87 | 5.97 | 6.8% |
| Sample 2 | 816 | 795 | 773 | 755 | 784 | 22.93 | 2.9% |
| Sample 3 | 1332 | 1482 | 1540 | 1536 | 1472 | 84.28 | 5.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1314-CAP|96-well plate precoated with anti- mouse CD48 antibody|1
EK1314-ST|Lyophilized recombinant mouse CD48 standard|10ng/tube×2
EK1314-DA|Biotinylated anti- mouse CD48 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
