Description
Boster’s mouse CD40L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD40L has been precoated onto 96-well plates. Standards(NSO, E61-L260) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD40L amount of sample captured in plate.
Synonyms: CD154|CD40 antigen ligand|CD40 ligand|CD40-L|CD40L_HUMAN|CD40LG|gp39|HIGM1|IGM|IMD3|soluble form|T B cell activating molecule|T BAM|T cell antigen Gp39|T-cell antigen Gp39|TNF related activation protein|TNF-related activation protein|TNFSF5|TrAP|Tumor necrosis factor (ligand) superfamily member 5|Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)|Tumor necrosis factor ligand superfamily member 5
Species: Mouse CD40L
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CD40L
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, E61-L260
Gene name: CD40 ligand / CD40 ligand
Protein name: CD40 ligand
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|202|698|1026|185|716|1058
Standard deviation|13.93|39.08|79|14.06|52.26|85.69
CV(%)|6.9%|5.6%|7.7%|7.6%|7.3%|8.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 202 | 213 | 180 | 201 | 199 | 11.93 | 5.9% |
| Sample 2 | 698 | 648 | 727 | 616 | 672 | 43.04 | 6.4% |
| Sample 3 | 1026 | 1040 | 977 | 1103 | 1036 | 44.95 | 4.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0574-CAP|96-well plate precoated with anti- mouse CD40L antibody|1
EK0574-ST|Lyophilized recombinant mouse CD40L standard|4ng/tubex2
EK0574-DA|Biotinylated anti- mouse CD40L antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
