Description
Boster’s mouse CA4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CA4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E18-S277) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CA4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Carbonic anhydrase 4; Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV; Ca4; Car4
Species: Mouse CA4
Assay: 78pg/ml-5000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant mouse CA4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, E18-S277
Gene name: Carbonic anhydrase 4 / carbonic anhydrase 4
Protein name: Carbonic anhydrase 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|135|612|2184|140|618|2258
Standard deviation|7.02|38.56|104.83|6.86|45.73|119.67
CV(%)|5.2%|6.3%|4.8%|4.9%|7.4%|5.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 135 | 147 | 139 | 153 | 143 | 6.98 | 4.8% |
| Sample 2 | 612 | 690 | 628 | 619 | 637 | 30.97 | 4.8% |
| Sample 3 | 2184 | 2286 | 2132 | 2361 | 2240 | 88.81 | 3.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1940-CAP|96-well plate precoated with anti- mouse CA4 antibody|1
EK1940-ST|Lyophilized recombinant mouse CA4 standard|10ng/tube×2
EK1940-DA|Biotinylated anti- mouse CA4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
