Description
Boster’s mouse SEZ6L ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SEZ6L has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A25-S301) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEZ6L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SEZ6L amount of sample captured in plate.
Synonyms: Carbonic anhydrase 12 (EC:4.2.1.1); Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Ca12; Car12
Species: Mouse CA12
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CA12
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, A25-S301
Gene name: Carbonic anhydrase 12 / carbonic anhydrase 12
Protein name: Carbonic anhydrase 12
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|24|105|321|28|111|358
Standard deviation|1.08|5.99|14.77|1.43|6.55|26.49
CV(%)|4.5%|5.7%|4.6%|5.1%|5.9%|7.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 24 | 24 | 25 | 24 | 24 | 0.43 | 1.7% |
| Sample 2 | 105 | 92 | 102 | 102 | 100 | 4.91 | 4.9% |
| Sample 3 | 321 | 275 | 281 | 288 | 291 | 17.78 | 6.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1833-CAP|96-well plate precoated with anti- mouse CA12 antibody|1
EK1833-ST|Lyophilized recombinant mouse CA12 standard|10ng/tube×2
EK1833-DA|Biotinylated anti- mouse CA12 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
