Description
Boster’s mouse CFD ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CFD has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: I26-S259) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CFD is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CFD amount of sample captured in plate.
Synonyms: Complement factor D; 28 kDa adipocyte protein; Adipsin; C3 convertase activator; Properdin factor D; Cfd; And; Df
Species: Mouse Adipsin/CFD
Assay: 0.78ng/ml-50ng/ml
Sensitivy: <20pg/ml Specifity: Natural and recombinant mouse CFD
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli,I26-S259
Gene name: Complement factor D / complement factor D (adipsin)
Protein name: Complement factor D
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1435|4423|19418|1328|4783|18803
Standard deviation|107.62|309.61|1378.67|111.55|411.33|1541.84
CV(%)|7.5%|7%|7.1%|8.4%|8.6%|8.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1435 | 1482 | 1554 | 1340 | 1452 | 77.67 | 5.3% |
| Sample 2 | 4423 | 4063 | 4082 | 3952 | 4130 | 176.29 | 4.2% |
| Sample 3 | 19418 | 20714 | 18137 | 20289 | 19639 | 985.26 | 5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity_x000D_
EK1420-CAP|96-well plate precoated with anti- mouse CFD antibody|1
EK1420-ST|Lyophilized recombinant mouse CFD standard|50ng/tube×2
EK1420-DA|Biotinylated anti- mouse CFD antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)_x000D_
AR1106-1|Sample diluent buffer|30ml_x000D_
AR1106-2|Antibody diluent buffer|12ml_x000D_
AR1106-3|ABC diluent buffer|12ml_x000D_
AR1104|TMB color developing agent|10ml_x000D_
AR1105|TMB stop solution|10ml_x000D_
PLA-SEA|Adhesive cover|4
