Description
Boster’s Monkey TNFSF13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF13 has been precoated onto 96-well plates. Standards (NSO,A105-L250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey TNFSF13 amount of sample captured in plate.
Synonyms: Tumor necrosis factor (Ligand) superfamily member 13 transcript variant delta ;Tumor necrosis factor ligand superfamily member 13 ;TNFSF13 ;
Species: Monkey TNFSF13
Assay: 156pg/ml-10,000pg/ml
Sensitivy: <10pg/ml Specifity:
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: Expression system for standard: NSO,A105-L250
Gene name: Tumor necrosis factor ligand superfamily member 13 / tumor necrosis factor (ligand) superfamily, member 13
Protein name: Tumor necrosis factor ligand superfamily member 13
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 653 | 2945 | 5633 | 864 | 3214 | 5366 |
| Standard deviation | 26.8 | 156.1 | 219.7 | 42.4 | 202.5 | 268.3 |
| CV(%) | 4.1 | 5.3 | 3.9 | 4.9 | 6.3 | 5 |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 264 | 265 | 272 | 285 | 271 | 8.38 | 3% |
| Sample 2 | 1700 | 1548 | 1563 | 1481 | 1573 | 79.55 | 5% |
| Sample 3 | 4235 | 4377 | 4466 | 4871 | 4487 | 236.37 | 5.2% |
*number of samples for each test n=16.
10ng/tube
