Antigen: a mixture of synthetic peptides corresponding two amino acids 268-282 and 337-352 of human MBD4 • Host: rabbit • Cross Reactivity: (+) human MBD4 • Application(s): ICC

MeCP2 Polyclonal Antibody – 1 ea

Brand:
Cayman
Storage:

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. A demethylase enzyme has been identified which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD).{17997} The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) repress transcription by binding specifically to methylated DNA.{17999} MBD2 and MBD4 (also known as MED1) colocalize with foci of heavily methylated satellite DNA and mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA either in vivo or in vitro. MeCP2, MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases.{17996} In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.{17995}  

 

Available on backorder

Add to quotes
SKU: 13775 - 1 ea Category:

Description

Antigen: a mixture of synthetic peptides corresponding two amino acids 268-282 and 337-352 of human MBD4 • Host: rabbit • Cross Reactivity: (+) human MBD4 • Application(s): ICC, IHC, and WB • MeCP2 may function as a mediator of the biological consequences of the methylation signal. It is also reported that this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Synonyms: Methyl-CpG-Binding Protein 2

Immunogen: mixture of synthetic peptides corresponding to amino acids 11-25 and 181-195 of human MeCP2

Formulation: 100 µg of protein G-purified IgG in 200 µl PBS containing 0.2% gelatin and 0.05% sodium azide

Isotype:

Applications: WB

Origin: Animal/Rabbit

Stability: 365 days

Application|Western Blot||Product Type|Antibodies|Polyclonal Antibodies||Research Area|Epigenetics, Transcription, & Translation|Readers||Research Area|Neuroscience

Related products