Description
Boster’s human TRAIL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TRAIL has been precoated onto 96-well plates. Standards (NSO,T95-G281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TRAIL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TRAIL amount of sample captured in plate.
Synonyms: Apo 2 ligand|_x000D_APO2L|_x000D_CD253|_x000D__x000D__x000D_Protein TRAIL|_x000D_TL2|_x000D_TNF related apoptosis inducing ligand|_x000D_Tumor necrosis factor ligand superfamily member 10
Species: Human TRAIL
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human TRAIL
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,T95-G281
Gene name: Tumor necrosis factor ligand superfamily member 10 / tumor necrosis factor (ligand) superfamily, member 10
Protein name: Tumor necrosis factor ligand superfamily member 10
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|38|184|416|34|175|377
Standard deviation|2.66|9.56|18.72|2.75|9.62|18.85
CV(%)|7%|5.2%|4.5%|8.1%|5.5%|5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 38 | 35 | 35 | 42 | 37 | 2.87 | 7.7% |
| Sample 2 | 184 | 187 | 185 | 189 | 186 | 1.92 | 1% |
| Sample 3 | 416 | 469 | 398 | 390 | 418 | 30.77 | 7.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0532-CAP|96-well plate precoated with anti- human TRAIL antibody|1
EK0532-ST|Lyophilized recombinant human TRAIL standard|10ng/tubex2
EK0532-DA|Biotinylated anti- human TRAIL antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
