Description
Boster’s human TIE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIE2 has been precoated onto 96-well plates. Standards(NSO, A23-K745) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIE2 amount of sample captured in plate.
Synonyms: Angiopoietin-1 receptor|_x000D_CD202b|_x000D_CD202b antigen|_x000D__x000D__x000D_EC 2.7.10.1|_x000D_hTIE2|_x000D_p140 TEK|_x000D_soluble TIE2 variant 1|_x000D_soluble TIE2 variant 2|_x000D_Tek|_x000D_TEK tyrosine kinase, endothelial|_x000D_TIE 2|_x000D_TIE2_HUMAN|_x000D_Tunica interna endothelial cell kinase|_x000D_Tyrosine-protein kinase receptor TEK|_x000D_Tyrosine-protein kinase receptor TIE 2|_x000D_Tyrosine-protein kinase receptor TIE-2|_x000D_venous malformations, multiple cutaneous and mucosal|_x000D_VMCM|_x000D_VMCM1
Species: Human TIE2
Assay: 156pg/ml-10000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant human TIE2
Cross Reactivity: There is cross-reactivates with human TIE1< 0.1%. Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, A23-K745
Gene name: Angiopoietin-1 receptor / TEK tyrosine kinase, endothelial
Protein name: Angiopoietin-1 receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|305|1860|4704|311|1714|4949
Standard deviation|23.79|81.84|263.42|27.05|99.41|351.37
CV(%)|7.8%|4.4%|5.6%|8.7%|5.8%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 305 | 289 | 298 | 271 | 290 | 12.73 | 4.3% |
| Sample 2 | 1860 | 1842 | 2015 | 1990 | 1926 | 76.52 | 3.9% |
| Sample 3 | 4704 | 5338 | 5060 | 5168 | 5067 | 232.08 | 4.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0519-CAP|96-well plate precoated with anti- human TIE2 antibody|1
EK0519-ST|Lyophilized recombinant human TIE2 standard|10ng/tubex2
EK0519-DA|Biotinylated anti- human TIE2 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
