Description
Boster’s human Thioredoxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Thioredoxin has been precoated onto 96-well plates. Standards(E.coli, V2-V105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Thioredoxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Thioredoxin amount of sample captured in plate.
Synonyms: ADF|ATL-derived factor|DKFZp686B1993|MGC61975|SASP|Surface associated sulphydryl protein|Surface-associated sulphydryl protein|THIO_HUMAN|Thioredoxin|TRDX|TRX 1|Trx|TRX1|TXN|TXN protein
Species: human Thioredoxin
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Thioredoxin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: E.coli, V2-V105
Gene name: Thioredoxin / thioredoxin
Protein name: Thioredoxin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|300|1212|4585|329|1301|4269
Standard deviation|18.6|83.62|183.4|20.72|89.76|204.91
CV(%)|6.2%|6.9%|4%|6.3%|6.9%|4.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 300 | 284 | 285 | 292 | 290 | 6.41 | 2.2% |
| Sample 2 | 1212 | 1056 | 1148 | 1119 | 1133 | 56.09 | 4.9% |
| Sample 3 | 4585 | 5257 | 4931 | 5111 | 4971 | 250.99 | 5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1254-CAP|96-well plate precoated with anti- human Thioredoxin antibody|1
EK1254-ST|Lyophilized recombinant human Thioredoxin standard|10ng/tube×2
EK1254-DA|Biotinylated anti- human Thioredoxin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
