Description
Boster’s human sVEGFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sVEGFR1 has been precoated onto 96-well plates. Standards(NSO, S27-H687) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sVEGFR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sVEGFR1 amount of sample captured in plate.
Synonyms: FLT 1 | FLT1 | Flt-1 | sflt 1 | sFLT1 | Soluble VEGFR1 variant 2 | Soluble VEGFR1 variant 21 | sVEGFR1 | Tyrosine protein kinase FRT | Tyrosine-protein kinase FRT | VEGF Receptor 1 | VEGFR 1 | VEGFR-1 | VGFR1
Species: Human sVEGFR1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <30pg/ml Specifity: Natural and recombinant human Svegfr1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: EDTA
Immunogen: NSO, S27-H687
Gene name: Vascular endothelial growth factor receptor 1 / fms-related tyrosine kinase 1
Protein name: Vascular endothelial growth factor receptor 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|237|1369|4420|240|1352|4316
Standard deviation|9.71|106.78|203.32|11.28|131.14|202.85
CV(%)|4.1%|7.8%|4.6%|4.7%|9.7%|4.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 237 | 212 | 228 | 228 | 226 | 9.01 | 3.9% |
| Sample 2 | 1369 | 1318 | 1326 | 1352 | 1341 | 20.36 | 1.5% |
| Sample 3 | 4420 | 4343 | 4598 | 4779 | 4535 | 168.51 | 3.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0543-CAP|96-well plate precoated with anti- human sVEGFR1 antibody|1
EK0543-ST|Lyophilized recombinant human sVEGFR1 standard|10ng/tubex2
EK0543-DA|Biotinylated anti- human sVEGFR1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
