Description
Boster’s human SPARCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPARCL1 has been precoated onto 96-well plates. Standards(NSO, I17-F664) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPARCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SPARCL1 amount of sample captured in plate.
Synonyms: Ecm2|Extracellular matrix protein 2|Hevin|High endothelial venule protein|MAST 9|mast9|Matrix glycoprotein Sc1|PIG33|proliferation inducing protein 33|RAGS1 antigen|SC1|SPARC like 1|SPARC-like protein 1|Sparcl1|SPRL1_HUMAN
Species: human SPARCL1
Assay: 46.9pg/ml-3000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human SPARCL1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, I17-F664
Gene name: SPARC-like protein 1 / SPARC-like 1 (hevin)
Protein name: SPARC-like protein 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|81|322|1261|78|330|1329
Standard deviation|4.45|17.71|79.44|4.75|22.11|94.35
CV(%)|5.5%|5.5%|6.3%|6.1%|6.7%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 81 | 81 | 84 | 89 | 83 | 3.26 | 3.9% |
| Sample 2 | 322 | 287 | 303 | 283 | 298 | 15.36 | 5.1% |
| Sample 3 | 1261 | 1280 | 1392 | 1332 | 1316 | 50.87 | 3.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1309-CAP|96-well plate precoated with anti- human SPARCL1 antibody|1
EK1309-ST|Lyophilized recombinant human SPARCL1 standard|10ng/tube×2
EK1309-DA|Biotinylated anti- human SPARCL1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
