Description
Boster’s human SP-D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for SP-D has been precoated onto 96-well plates. Standards(NSO, A21-F375) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SP-D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SP-D amount of sample captured in plate.
Synonyms: COLEC 7|COLEC7|Collectin-7|Collectin7|Lung surfactant protein D|PSP D|PSP-D|PSP-D Surfactant protein D|PSPD|Pulmonary surfactant apoprotein|Pulmonary surfactant associated protein D|Pulmonary surfactant associated protein PSP-D|Pulmonary surfactant-associated protein D|SFTP 4|SFTP4|SFTPD|SFTPD_HUMAN|SP D|SP-D|Surfactant associated protein pulmonary 4|Surfactant pulmonary associated protein D
Species: human SP-D
Assay: 625pg/ml-40000pg/ml
Sensitivy: <20pg/ml Specifity: Natural and recombinant human SP-D
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, A21-F375
Gene name: Pulmonary surfactant-associated protein D / surfactant protein D
Protein name: Pulmonary surfactant-associated protein D
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1547|3580|25269|1591|3588|23717
Standard deviation|92.82|221.96|1693.02|111.37|279.86|1683.9
CV(%)|6%|6.2%|6.7%|7%|7.8%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1547 | 1291 | 1390 | 1463 | 1422 | 94.19 | 6.6% |
| Sample 2 | 3580 | 3482 | 3689 | 3752 | 3625 | 103.31 | 2.8% |
| Sample 3 | 25269 | 29048 | 29153 | 26765 | 27558 | 1630.37 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1171-CAP|96-well plate precoated with anti- human SP-D antibody|1
EK1171-ST|Lyophilized recombinant human SP-D standard|40ng/tube×2
EK1171-DA|Biotinylated anti- human SP-D antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
