Description
Boster’s human SEZ6L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SEZ6L has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L29-I1024) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEZ6L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Seizure 6-like protein; SEZ6L; KIAA0927; UNQ2542/PRO6094
Species: Human SEZ6L
Assay: 0.312ng/ml-20ng/ml
Sensitivy: <0.1ng/ml Specifity: Natural and recombinant human SEZ6L
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, L29-I1024
Gene name: Seizure 6-like protein / seizure related 6 homolog like
Protein name: Seizure 6-like protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|489|1734|10155|544|2012|11046
Standard deviation|31.30|97.10|710.85|23.39|114.68|839.50
CV(%)|6.4%|5.6%|7.0%|4.3%|5.7%|7.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 489 | 478 | 517 | 524 | 502 | 19.06 | 3.7% |
| Sample 2 | 1734 | 1756 | 1906 | 1797 | 1798 | 66.19 | 3.6% |
| Sample 3 | 10155 | 8845 | 9766 | 8917 | 9420 | 557.57 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1862-CAP|96-well plate precoated with anti- human SEZ6L antibody|1
EK1862-ST|Lyophilized recombinant human SEZ6L standard|20ng/tube×2
EK1862-DA|Biotinylated anti- human SEZ6L antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
