Description
Boster’s human S100A12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for S100A12 has been precoated onto 96-well plates. Standards(E.coli,M1-E92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human S100A12 amount of sample captured in plate.
Synonyms: CAAF1|CAGC|Calcitermin|Calcium binding protein in amniotic fluid|Calcium-binding protein in amniotic fluid 1|Calgranulin C|Calgranulin related protein|Calgranulin-C|Calgranulin-related protein|CGRP|EN RAGE|EN-RAGE|ENRAGE|Extracellular newly identified RAGE-binding protein|migration inhibitory factor-related protein 6|MRP6|Neutrophil S100 protein|p6|Protein S100 A12|S100 calcium binding protein A12|S100 calcium-binding protein A12 (calgranulin C)|S100 calcium-binding protein A12|S100A12|S10AC_HUMAN
Species: human S100A12
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human S100A12
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, M1-E92
Gene name: Protein S100-A12 / S100 calcium binding protein A12
Protein name: Protein S100-A12
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|58|265|947|56|247|855
Standard deviation|2.32|20.4|67.23|3.08|23.46|68.4
CV(%)|4%|7.7%|7.1%|5.5%|9.5%|8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 61 | 58 | 60 | 59 | 1.29 | 2.1% |
| Sample 2 | 265 | 274 | 252 | 278 | 267 | 9.98 | 3.7% |
| Sample 3 | 947 | 981 | 966 | 1077 | 992 | 50.11 | 5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1169-CAP|96-well plate precoated with anti- human S100A12 antibody|1
EK1169-ST|Lyophilized recombinant human S100A12 standard|10ng/tube×2
EK1169-DA|Biotinylated anti- human S100A12 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
