Description
Boster’s human Renin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Renin has been precoated onto 96-well plates. Standards(NSO, L67-R406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Renin amount of sample captured in plate.
Synonyms: ATP6AP2|ATP6M8-9|ATPase H(+)-transporting lysosomal accessory protein 2|ATPase H(+)-transporting lysosomal-interacting protein 2|Embryonic liver differentiation factor 10|ER-localized type I transmembrane adaptor|N14F|Renin receptor|Renin/prorenin receptor|RENR_HUMAN|V-ATPase M8.9 subunit|Vacuolar ATP synthase membrane sector-associated protein M8-9
Species: human Renin
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Renin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, L67-R406
Gene name: Renin / renin
Protein name: Renin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|73|224|875|68|211|909
Standard deviation|4.52|13.66|56|4.69|16.88|71.81
CV(%)|6.2%|6.1%|6.4%|6.9%|8%|7.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 73 | 79 | 69 | 73 | 73 | 3.57 | 4.8% |
| Sample 2 | 224 | 224 | 208 | 232 | 222 | 8.71 | 3.9% |
| Sample 3 | 875 | 929 | 953 | 900 | 914 | 29.42 | 3.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1249-CAP|96-well plate precoated with anti- human Renin antibody|1
EK1249-ST|Lyophilized recombinant human Renin standard|10ng/tube×2
EK1249-DA|Biotinylated anti- human Renin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
