Description
Boster’s human REG1A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for REG1A has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q23-N166) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for REG1A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Lithostathine-1-alpha; Islet cells regeneration factor; ICRF; Islet of Langerhans regenerating protein; REG; Pancreatic stone protein; PSP; Pancreatic thread protein; PTP; Regenerating islet-derived protein 1-alpha; REG-1-alpha; Regenerating protein I alpha; REG1A; PSPS1
Species: Human REG1A
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human REG1A
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, Q23-N166
Gene name: Lithostathine-1-alpha / regenerating family member 1 alpha
Protein name: Lithostathine-1-alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|89|786|1169|121|845|1307
Standard deviation|4.63|51.09|66.63|8.35|49.01|82.34
CV(%)|5.2%|6.5%|5.7%|6.9%|5.8%|6.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 87 | 91 | 91 | 87 | 89 | 2.00 | 2.2% |
| Sample 2 | 790 | 831 | 781 | 745 | 786 | 30.59 | 3.8% |
| Sample 3 | 1112 | 1254 | 1221 | 1089 | 1169 | 69.96 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1812-CAP|96-well plate precoated with anti- human REG1A antibody|1
EK1812-ST|Lyophilized recombinant human REG1A standard|10ng/tube×2
EK1812-DA|Biotinylated anti- human REG1A antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
