Description
Boster’s human PEDF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for PEDF has been precoated onto 96-well plates. Standards(NSO, M1-P418) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PEDF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PEDF amount of sample captured in plate.
Synonyms: Alpha 2 antiplasmin|Cell proliferation-inducing gene 35 protein|EPC 1|EPC-1|EPC1|OI12|OI6|PEDF|PEDF_HUMAN|PIG 35|PIG35|Pigment epithelium derived factor|Pigment epithelium-derived factor|Proliferation inducing protein 35|serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1|SERPIN F1|Serpin peptidase inhibitor clade F member 1|serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1|SERPINF 1|SERPINF1
Species: Human PEDF
Assay: 0.78ng/ml-50ng/ml
Sensitivy: <20pg/ml Specifity: Natural and recombinant human PEDF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, M1-P418
Gene name: Pigment epithelium-derived factor / serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Protein name: Pigment epithelium-derived factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1135|5918|20509|1124|5439|18927
Standard deviation|76.04|337.32|820.36|80.92|364.41|908.49
CV(%)|6.7%|5.7%|4%|7.2%|6.7%|4.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1135 | 1046 | 1156 | 1186 | 1130 | 52.17 | 4.6% |
| Sample 2 | 5918 | 6122 | 5459 | 5973 | 5868 | 247.64 | 4.2% |
| Sample 3 | 20509 | 19679 | 21913 | 20356 | 20614 | 812.28 | 3.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0896-CAP|96-well plate precoated with anti- human PEDF antibody|1
EK0896-ST|Lyophilized recombinant human PEDF standard|50ng/tube×2
EK0896-DA|Biotinylated anti- human PEDF antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
