Description
Boster’s human PECAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PECAM-1 has been precoated onto 96-well plates. Standards(CHO, Q28-K601) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PECAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PECAM-1 amount of sample captured in plate.
Synonyms: CD31|CD31 antigen|EndoCAM|GPIIA|HEC7|Leukocyte surface protein|PECA1|PECA1_HUMAN|PECAM 1|PECAM-1|Pecam1|Platelet endothelial cell adhesion molecule|Platelet/endothelial cell adhesion molecule
Species: Human PECAM-1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human PECAM-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: CHO, Q28-K601
Gene name: Platelet endothelial cell adhesion molecule / platelet/endothelial cell adhesion molecule 1f
Protein name: Platelet endothelial cell adhesion molecule
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|292|1327|4781|278|1442|4709
Standard deviation|11.68|87.58|310.76|13.62|118.24|367.3
CV(%)|4%|6.6%|6.5%|4.9%|8.2%|7.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 292 | 286 | 327 | 276 | 295 | 19.2 | 6.5% |
| Sample 2 | 1327 | 1428 | 1563 | 1332 | 1412 | 95.76 | 6.7% |
| Sample 3 | 4781 | 4159 | 4685 | 4186 | 4452 | 282.45 | 6.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0873-CAP|96-well plate precoated with anti- human PECAM-1 antibody|1
EK0873-ST|Lyophilized recombinant human PECAM-1 standard|10ng/tube×2
EK0873-DA|Biotinylated anti- human PECAM-1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
