Description
Boster’s human PD-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for PD-1 has been precoated onto 96-well plates. Standards(NSO, L25-Q167) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PD-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PD-1 amount of sample captured in plate.
Synonyms: CD279|CD279 antigen|hPD 1|hPD-1|PD 1|PD-1|PD1|PDCD 1|PDCD1|PDCD1_HUMAN|Programmed cell death 1|Programmed cell death protein 1|Protein PD 1|Protein PD-1|SLEB2
Species: Human PD-1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human PD-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, L25-Q167
Gene name: Programmed cell death protein 1 / programmed cell death 1
Protein name: Programmed cell death protein 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|319|1114|4321|316|1080|4600
Standard deviation|13.39|56.81|224.69|16.11|56.16|289.8
CV(%)|4.2%|5.1%|5.2%|5.1%|5.2%|6.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 319 | 311 | 321 | 328 | 319 | 6.05 | 1.8% |
| Sample 2 | 1114 | 1170 | 1172 | 1200 | 1164 | 31.2 | 2.6% |
| Sample 3 | 4321 | 4232 | 3750 | 4429 | 4183 | 259.54 | 6.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0959-CAP|96-well plate precoated with anti- human PD-1 antibody|1
EK0959-ST|Lyophilized recombinant human PD-1 standard|10ng/tube×2
EK0959-DA|Biotinylated anti- human PD-1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
