Description
Boster’s human PCSK9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PCSK9 has been precoated onto 96-well plates. Standards(NSO, S153-Q692) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PCSK9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PCSK9 amount of sample captured in plate.
Synonyms: Convertase subtilisin/kexin type 9 preproprotein|FH3|HCHOLA3|Hypercholesterolemia autosomal dominant 3|LDLCQ1|NARC 1|NARC-1|NARC1|Neural apoptosis regulated convertase 1|Neural apoptosis-regulated convertase 1|PC 9|PC9|PCSK 9|PCSK9|PCSK9_HUMAN|Proprotein convertase 9|Proprotein convertase PC9|Proprotein convertase subtilisin/kexin type 9|PSEC0052|Subtilisin/kexin like protease PC9|Subtilisin/kexin-like protease PC9
Species: human PCSK9
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human PCSK9
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S153-Q692
Gene name: Proprotein convertase subtilisin/kexin type 9 / proprotein convertase subtilisin/kexin type 9
Protein name: Proprotein convertase subtilisin/kexin type 9
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|229|1991|5404|215|1927|5944
Standard deviation|16.03|125.43|243.18|17.63|132.96|285.31
CV(%)|7%|6.3%|4.5%|8.2%|6.9%|4.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 229 | 229 | 219 | 191 | 217 | 15.55 | 7.1% |
| Sample 2 | 1991 | 1900 | 1655 | 1887 | 1858 | 123.99 | 6.6% |
| Sample 3 | 5404 | 4654 | 4674 | 4865 | 4899 | 302.83 | 6.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1147-CAP|96-well plate precoated with anti- human PCSK9 antibody|1
EK1147-ST|Lyophilized recombinant human PCSK9 standard|10ng/tube×2
EK1147-DA|Biotinylated anti- human PCSK9 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
