Description
Boster’s human PCOLCE ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PCOLCE has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q26-D449) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PCOLCE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PCOLCE amount of sample captured in plate.
Synonyms: Procollagen C-endopeptidase enhancer 1; Procollagen COOH-terminal proteinase enhancer 1; PCPE-1; Procollagen C-proteinase enhancer 1; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer; PCOLCE; PCPE1
Species: Human PCOLCE
Assay: 156pg/ml-10000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant human PCOLCE
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated monoclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Q26-D449
Gene name: Procollagen C-endopeptidase enhancer 1 / procollagen C-endopeptidase enhancer
Protein name: Procollagen C-endopeptidase enhancer 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|242|1644|5631|217|1771|5410
Standard deviation|14.52|118.36|360.38|14.53|146.99|394.93
CV(%)|6%|7.2%|6.4%|6.7%|8.3%|7.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 242 | 247 | 246 | 242 | 244 | 2.27 | 0.9% |
| Sample 2 | 1644 | 1884 | 1788 | 1601 | 1729 | 113.04 | 6.5% |
| Sample 3 | 5631 | 5520 | 5145 | 4973 | 5317 | 268.19 | 5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK2105-CAP|96-well plate precoated with anti- human PCOLCE antibody|1
EK2105-ST|Lyophilized recombinant human PCOLCE standard|10ng/tube×2
EK2105-DA|Biotinylated anti- human PCOLCE antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
