Description
Boster’s human MICB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MICB has been precoated onto 96-well plates. Standards(NSO, A23-G298) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MICB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MICB amount of sample captured in plate.
Synonyms: MHC class I chain related protein B|_x000D_MHC class I like molecule PERB11.2 IMX|_x000D_MHC class I mic B antigen|_x000D__x000D__x000D_MHC class I polypeptide related sequence B|_x000D_MHC class I polypeptide-related sequence B|_x000D_MIC B|_x000D_MIC-B|_x000D_MICB|_x000D_MICB_HUMAN|_x000D_PERB11.2|_x000D_Stress inducible class I homolog
Species: Human MICB
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human MICB
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, A23-G298
Gene name: MHC class I polypeptide-related sequence B / MHC class I polypeptide-related sequence B
Protein name: MHC class I polypeptide-related sequence B
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|174|694|1403|180|758|1442
Standard deviation|9.91|50.66|78.56|12.24|56.85|82.19
CV(%)|5.7%|7.3%|5.6%|6.8%|7.5%|5.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 174 | 167 | 176 | 157 | 168 | 7.43 | 4.4% |
| Sample 2 | 694 | 670 | 590 | 703 | 664 | 44.53 | 6.7% |
| Sample 3 | 1403 | 1209 | 1282 | 1179 | 1268 | 86.34 | 6.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0963-CAP|96-well plate precoated with anti- human MICB antibody|1
EK0963-ST|Lyophilized recombinant human MICB standard|10ng/tube×2
EK0963-DA|Biotinylated anti- human MICB antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
