Description
Boster’s human MCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MCAM has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: V24-G559) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MCAM amount of sample captured in plate.
Synonyms: Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD146; MCAM; MUC18
Species: Human MCAM
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human MCAM
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO,V24-G559
Gene name: Cell surface glycoprotein MUC18 / melanoma cell adhesion molecule
Protein name: Cell surface glycoprotein MUC18
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|143|548|1021|131|561|1060
Standard deviation|6|23.56|62.28|7.07|31.97|80.56
CV(%)|4.2%|4.3%|6.1%|5.4%|5.7%|7.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 143 | 158 | 162 | 156 | 154 | 7.11 | 4.6% |
| Sample 2 | 548 | 529 | 527 | 526 | 532 | 9.01 | 1.6% |
| Sample 3 | 1021 | 1095 | 1036 | 1044 | 1049 | 27.81 | 2.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1675-CAP|96-well plate precoated with anti- human MCAM antibody|1
EK1675-ST|Lyophilized recombinant human MCAM standard|4ng/tube×2
EK1675-DA|Biotinylated anti- human MCAM antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
