Description
Boster’s human LOX-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LOX-1 has been precoated onto 96-well plates. Standards(NSO, S61-Q273) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOX-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LOX-1 amount of sample captured in plate.
Synonyms: C-type lectin domain family 8 member A|CLEC8A|hLOX-1|Lectin-like oxidized LDL receptor 1|Lectin-like oxLDL receptor 1|Lectin-type oxidized LDL receptor 1|LOX-1|LOXIN|Olr1|OLR1_HUMAN|Ox-LDL receptor 1|Oxidized low density lipoprotein (lectin like) receptor 1|Oxidized low density lipoprotein receptor 1 soluble form|Oxidized low-density lipoprotein receptor 1|SCARE1|Scavenger receptor class E member 1|SLOX1
Species: Human LOX-1
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human LOX-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S61-Q273
Gene name: Oxidized low-density lipoprotein receptor 1 / oxidized low density lipoprotein (lectin-like) receptor 1
Protein name: Oxidized low-density lipoprotein receptor 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|88|211|833|90|192|784
Standard deviation|6.86|12.02|64.14|8.46|10.94|69.77
CV(%)|7.8%|5.7%|7.7%|9.4%|5.7%|8.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 88 | 77 | 81 | 83 | 82 | 3.96 | 4.8% |
| Sample 2 | 211 | 220 | 195 | 196 | 205 | 10.5 | 5.1% |
| Sample 3 | 833 | 881 | 979 | 973 | 916 | 61.9 | 6.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0824-CAP|96-well plate precoated with anti- human LOX-1 antibody|1
EK0824-ST|Lyophilized recombinant human LOX-1 standard|10ng/tube×2
EK0824-DA|Biotinylated anti- human LOX-1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
