Description
Boster’s human Livin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Livin has been precoated onto 96-well plates. Standards(E.coli, M1-S298) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Livin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Livin amount of sample captured in plate.
Synonyms: Baculoviral IAP repeat containing 7|Baculoviral IAP repeat containing protein 7|Baculoviral IAP repeat-containing protein 7|BIRC 7|BIRC7|BIRC7_HUMAN|KIAP|Kidney inhibitor of apoptosis protein|LIVIN|Livin inhibitor of apotosis|Melanoma inhibitor of apoptosis protein|ML IAP|ML-IAP|MLIAP|RING finger protein 50|RNF 50|RNF50
Species: human Livin
Assay: 0.78ng/ml-50ng/ml
Sensitivy: <20pg/ml Specifity: Natural and recombinant human Livin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: E.coli, M1-S298
Gene name: Baculoviral IAP repeat-containing protein 7 / baculoviral IAP repeat containing 7
Protein name: Baculoviral IAP repeat-containing protein 7
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1179|7213|23834|1141|6671|22267
Standard deviation|75.45|310.15|1430.04|76.44|353.56|1625.49
CV(%)|6.4%|4.3%|6%|6.7%|5.3%|7.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1179 | 1307 | 1210 | 1282 | 1244 | 51.94 | 4.1% |
| Sample 2 | 7213 | 6992 | 7670 | 6636 | 7127 | 374.68 | 5.2% |
| Sample 3 | 23834 | 20813 | 23219 | 23818 | 22921 | 1242.03 | 5.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1348-CAP|96-well plate precoated with anti- human Livin antibody|1
EK1348-ST|Lyophilized recombinant human Livin standard|100ng/tube×2
EK1348-DA|Biotinylated anti- human Livin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
