Description
Boster’s human IL1R1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL1R1 has been precoated onto 96-well plates. Standards(NSO, D21-T332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R1 amount of sample captured in plate.
Synonyms: IL-1R-1|IL-1RT-1|IL-1RT1|CD121 antigen-like family member A|Interleukin-1 receptor alpha|IL-1R-alpha|Interleukin-1 receptor type I|p80|CD_antigen: CD121a|L1R|IL1RA|IL1RT1|CD121A|D2S1473
Species: Human IL1R1
Assay: 125pg/ml-8000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human IL1R1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, D21-T332
Gene name: Interleukin-1 receptor type 1 / interleukin 1 receptor, type I
Protein name: Interleukin-1 receptor type 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|188|973|4770|205|1005|4361
Standard deviation|8.46|63.24|195.57|10.86|77.38|248.57
CV(%)|4.5%|6.5%|4.1%|5.3%|7.7%|5.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 188 | 179 | 191 | 173 | 182 | 7.15 | 3.9% |
| Sample 2 | 973 | 977 | 935 | 964 | 962 | 16.42 | 1.7% |
| Sample 3 | 4770 | 4715 | 5460 | 5318 | 5065 | 327.7 | 6.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0395-CAP|96-well plate precoated with anti- human IL1R1 antibody|1
EK0395-ST|Lyophilized recombinant human IL1R1 standard|10ng/tubex2
EK0395-DA|Biotinylated anti- human IL1R1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
