Description
Boster’s human IL-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-8 has been precoated onto 96-well plates. Standards(E.coli, S28-S99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-8 amount of sample captured in plate.
Synonyms: (Ala-IL-8)77|_x000D_(Ser-IL-8)72|_x000D_3 10C|_x000D__x000D__x000D_3-10C|_x000D_AMCF I|_x000D_AMCF-I|_x000D_AMCFI|_x000D_b ENAP|_x000D_B-ENAP|_x000D_Beta-thromboglobulin-like protein|_x000D_C X C motif chemokine 8|_x000D_C-X-C motif chemokine 8|_x000D_Chemokine (C X C motif) ligand 8|_x000D_CXCL8 |_x000D_Emoctakin|_x000D_GCP 1|_x000D_GCP-1|_x000D_GCP/IL-8 protein I|_x000D_GCP/IL-8 protein II|_x000D_GCP/IL-8 protein III|_x000D_GCP/IL-8 protein IV|_x000D_GCP/IL-8 protein V|_x000D_GCP/IL-8 protein VI|_x000D_GCP1 |_x000D_Granulocyte chemotactic protein 1|_x000D_IL 8|_x000D_IL-8|_x000D_IL-8(1-77)|_x000D_IL-8(9-77)|_x000D_IL8|_x000D_IL8/NAP1 form I|_x000D_IL8/NAP1 form II|_x000D_IL8/NAP1 form III|_x000D_IL8/NAP1 form IV|_x000D_IL8/NAP1 form V|_x000D_IL8/NAP1 form VI|_x000D_IL8_HUMAN|_x000D_Interleukin 8|_x000D_K60|_x000D_LECT|_x000D_LUCT|_x000D_Lymphocyte derived neutrophil activating factor|_x000D_Lymphocyte-derived neutrophil-activating factor|_x000D_LYNAP|_x000D_MDNCF|_x000D_MDNCF-b|_x000D_MDNCF-c|_x000D_MONAP|_x000D_Monocyte derived neutrophil activating peptide|_x000D_Monocyte derived neutrophil chemotactic factor|_x000D_Monocyte-derived neutrophil chemotactic factor|_x000D_Monocyte-derived neutrophil-activating peptide|_x000D_NAF|_x000D_NAP 1|_x000D_NAP-1|_x000D_NAP1|_x000D_Neutrophil activating peptide 1|_x000D_Neutrophil activating protein 1|_x000D_Neutrophil-activating factor|_x000D_Neutrophil-activating protein 1|_x000D_Protein 3 10C|_x000D_Protein 3-10C|_x000D_SCYB8|_x000D_Small inducible cytokine subfamily B member 8|_x000D_T cell chemotactic factor|_x000D_T-cell chemotactic factor|_x000D_TSG 1|_x000D_TSG-1|_x000D_TSG1
Species: Human IL-8
Assay: 15.6pg/ml-1000pg/ml(cellculturesupernates)7.8pg/ml-500pg/ml(serum,plasma)
Sensitivy: <1pg/ml Specifity: Natural and recombinant human IL-8
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, S28-S99
Gene name: Interleukin-8 / chemokine (C-X-C motif) ligand 8
Protein name: Interleukin-8
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|16|66|234|16|63|230
Standard deviation|0.72|2.83|14.27|0.8|3.84|17.48
CV(%)|4.5%|4.3%|6.1%|5%|6.1%|7.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 16 | 16 | 15 | 17 | 16 | 0.7 | 4.3% |
| Sample 2 | 66 | 67 | 72 | 74 | 69 | 3.34 | 4.8% |
| Sample 3 | 234 | 249 | 255 | 221 | 239 | 13.25 | 5.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0413-CAP|96-well plate precoated with anti- human IL-8 antibody|1
EK0413-ST|Lyophilized recombinant human IL-8 standard|10ng/tubex2
EK0413-DA|Biotinylated anti- human IL-8 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
