Description
Boster’s human IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-5 has been precoated onto 96-well plates. Standards(sf21, I20-S134) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-5 amount of sample captured in plate.
Synonyms: Interleukin-5;IL-5;B-cell differentiation factor I;Eosinophil differentiation factor;T-cell replacing factor;TRF;IL5;
Species: Human IL-5
Assay: 7.8pg/ml-500pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant human IL-5
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: Expression system for standard: sf21; Immunogen sequence: I20-S134
Gene name: Interleukin-5 / Interleukin-5
Protein name: Interleukin-5
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 9 | 73 | 238 | 8 | 70 | 234 |
| Standard deviation | 0.7 | 4.67 | 16.42 | 0.74 | 4.55 | 20.12 |
| CV(%) | 7.8% | 6.4% | 6.9% | 9.3% | 6.5% | 8.6% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 9 | 10 | 10 | 9 | 9 | 0.5 | 5.5% |
| Sample 2 | 73 | 73 | 74 | 62 | 70 | 4.92 | 7% |
| Sample 3 | 238 | 246 | 219 | 235 | 234 | 9.81 | 4.1% |
*number of samples for each test n=16.
10ng/tube
