Description
Boster’s human IL-28A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-28A has been precoated onto 96-well plates. Standards(NSO, V26-V200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-28A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-28A amount of sample captured in plate.
Synonyms: Cytokine ZCYTO20|_x000D_EG330496|_x000D_IFN-lambda-2|_x000D__x000D__x000D_IFNL2|_x000D_IL 28A|_x000D_IL-28A|_x000D_IL28A|_x000D_IL28A_HUMAN|_x000D_Interferon lambda 2|_x000D_Interferon lambda-2|_x000D_Interleukin 28A|_x000D_Interleukin-28A
Species: Human IL-28A
Assay: 125pg/ml-8000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human IL-28A
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, V26-V200
Gene name: Interferon lambda-2 / interferon, lambda 2
Protein name: Interferon lambda-2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|162|1227|4870|153|1196|5054
Standard deviation|6.8|67.48|282.46|8.26|78.93|343.67
CV(%)|4.2%|5.5%|5.8%|5.4%|6.6%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 162 | 184 | 181 | 182 | 177 | 8.87 | 5% |
| Sample 2 | 1227 | 1413 | 1423 | 1411 | 1368 | 81.82 | 5.9% |
| Sample 3 | 4870 | 5325 | 4875 | 4906 | 4994 | 191.59 | 3.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0969-CAP|96-well plate precoated with anti- human IL-28A antibody|1
EK0969-ST|Lyophilized recombinant human IL-28A standard|10ng/tube×2
EK0969-DA|Biotinylated anti- human IL-28A antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
