Human IL-1RA PicoKine ELISA Kit

Brand:
Boster Bio
Storage:

For quantitative detection of human IL-1RA in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

 

Available on backorder

SKU: EK0782 Category:

Description

Boster’s human IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli, R26-E177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1RA amount of sample captured in plate.


Synonyms: Anakinra|DIRA|ICIL 1RA|ICIL-1RA|IL 1ra|IL 1RN|IL-1ra|IL-1RN|IL1 inhibitor|IL1F3|IL1RA|IL1RA_HUMAN|IL1RN|Interleukin 1 receptor antagonist|Interleukin 1 receptor antagonist protein|Interleukin-1 receptor antagonist protein|Intracellular IL 1 receptor antagonist type II|Intracellular interleukin 1 receptor antagonist|IRAP|MGC10430|MVCD4|Type II interleukin 1 receptor antagonist

Species: Human IL-1RA

Assay: 31.2pg/ml-2000pg/ml

Sensitivy: <2pg/ml Specifity: Natural and recombinant human IL-1RA

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Anticoagulant: heparin or EDTA

Immunogen: E.coli, R26-E177

Gene name: Interleukin-1 receptor antagonist protein / interleukin 1 receptor antagonist

Protein name: Interleukin-1 receptor antagonist protein

Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)


|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|57|296|803|56|318|731
Standard deviation|3.59|20.42|42.55|4.2|27.34|48.97
CV(%)|6.3%|6.9%|5.3%|7.5%|8.6%|6.7%


Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 57 52 59 58 56 2.69 4.8%
Sample 2 296 274 294 294 289 8.98 3.1%
Sample 3 803 845 834 932 853 47.86 5.6%

*number of samples for each test n=16.


Catalog number|Description|Quantity
EK0782-CAP|96-well plate precoated with anti- human IL-1RA antibody|1
EK0782-ST|Lyophilized recombinant human IL-1RA standard|10ng/tube×2
EK0782-DA|Biotinylated anti- human IL-1RA antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4