Description
Boster’s human GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDF-15 has been precoated onto 96-well plates. Standards(CHO, A197-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDF-15 amount of sample captured in plate.
Synonyms: Growth/differentiation factor 15;GDF-15;Macrophage inhibitory cytokine 1;MIC-1;NSAID-activated gene 1 protein;NAG-1;NSAID-regulated gene 1 protein;NRG-1;Placental TGF-beta;Placental bone morphogenetic protein;Prostate differentiation factor;GDF15;MIC1, PDF, PLAB, PTGFB;
Species: Human GDF-15
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human GDF-15
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: Expression system for standard: CHO; Immunogen sequence: A197-I308
Gene name: Growth/differentiation factor 15 / Growth/differentiation factor 15
Protein name: Growth/differentiation factor 15
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 53 | 252 | 1068 | 53 | 266 | 1115 |
| Standard deviation | 3.55 | 16.12 | 74.76 | 3.92 | 20.21 | 81.39 |
| CV(%) | 6.7% | 6.4% | 7% | 7.4% | 7.6% | 7.3% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 53 | 56 | 49 | 57 | 53 | 3.11 | 5.8% |
| Sample 2 | 252 | 245 | 249 | 271 | 254 | 9.98 | 3.9% |
| Sample 3 | 1068 | 1033 | 966 | 984 | 1012 | 40.23 | 3.9% |
*number of samples for each test n=16.
10ng/tube
