Description
Boster’s human ERBB3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ERBB3 has been precoated onto 96-well plates. Standards (NSO, S20-T643) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ERBB3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ERBB3 amount of sample captured in plate.
Synonyms: c erbB 3|c erbB3|Erb b2 receptor tyrosine kinase 3|ErbB 3|ERBB3|ERBB3 protein|erbB3 S|ERBB3_HUMAN|Glial growth factor receptor|HER 3|
HER3|Human epidermal growth factor receptor 3|LCCS2|MDA BF 1|MGC88033|p180 ErbB3|p45 sErbB3|p85 sErbB3|proto-oncogene-like protein c ErbB 3|proto-oncogene-like protein c ErbB3|Proto-oncogene-like protein c-ErbB-3|Receptor tyrosine protein kinase erbB 3|Receptor tyrosine protein kinase erbB3|Receptor tyrosine-protein kinase erbB-3|Tyrosine kinase type cell surface receptor HER3|Tyrosine kinase-type cell surface receptor HER3|v erb b2 avian erythroblastic leukemia viral oncogene homolog 3|v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian)|v erb b2 erythroblastic leukemia viral oncogene homolog 3
Species: human ERBB3
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human ERBB3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S20-T643
Gene name: Receptor tyrosine-protein kinase erbB-3 / erb-b2 receptor tyrosine kinase 3
Protein name: Receptor tyrosine-protein kinase erbB-3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|249|1903|5498|238|1734|5885
Standard deviation|12.45|125.59|324.38|16.42|114.44|406.06
CV(%)|5%|6.6%|5.9%|6.9%|6.6%|6.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 249 | 249 | 242 | 239 | 244 | 4.38 | 1.7% |
| Sample 2 | 1903 | 1635 | 1831 | 1793 | 1790 | 98.08 | 5.4% |
| Sample 3 | 5498 | 5793 | 5614 | 5244 | 5537 | 199.27 | 3.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1398-CAP|96-well plate precoated with anti- human ERBB3 antibody|1
EK1398-ST|Lyophilized recombinant human ERBB3 standard|10ng/tube×2
EK1398-DA|Biotinylated anti- human ERBB3 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30|ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
