Description
Boster’s human Emmprin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Emmprin has been precoated onto 96-well plates. Standards(NSO, T25-H205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Emmprin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Emmprin amount of sample captured in plate.
Synonyms: 5F7|_x000D_BASI_HUMAN|_x000D_Basigin|_x000D__x000D__x000D_Bsg|_x000D_CD147|_x000D_Collagenase stimulatory factor|_x000D_EMMPRIN|_x000D_Extracellular matrix metalloproteinase inducer|_x000D_Leukocyte activation antigen M6|_x000D_OK blood group antigen|_x000D_TCSF|_x000D_Tumor cell-derived collagenase stimulatory factor
Species: Human Emmprin
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant human Emmprin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, T25-H205
Gene name: Basigin / basigin (Ok blood group)
Protein name: Basigin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|69|232|1065|70|210|975
Standard deviation|5.24|16.24|76.68|5.32|15.54|77.02
CV(%)|7.6%|7%|7.2%|7.6%|7.4%|7.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 69 | 69 | 64 | 65 | 66 | 2.27 | 3.4% |
| Sample 2 | 232 | 198 | 221 | 210 | 215 | 12.63 | 5.8% |
| Sample 3 | 1065 | 992 | 955 | 1022 | 1008 | 40.33 | 4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0751-CAP|96-well plate precoated with anti- human Emmprin antibody|1
EK0751-ST|Lyophilized recombinant human Emmprin standard|10ng/tube×2
EK0751-DA|Biotinylated anti- human Emmprin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
